Figure 1From: In vitro gene silencing of independent phosphoglycerate mutase (iPGM) in the filarial parasite Brugia malayiConfirmation of penetration of siRNA to mf, L3, and adult female worms. The Cy3-labeled negative control siRNA was taken up by all three stages of parasite in vitro after soaking for 24 hours at 2 μM concentration. The fluorescence was visualized using a Nikon fluorescence microscope using the rhodamine filter set at emission 590 nm.Back to article page