Figure 5

The percent reduction in gene transcript level in Bm-ipgm specific siRNA treated worms. Two female worms at different time intervals (12, 24, 36, 48, 60 hours) and 48 hours after transfer to normal siRNA-free culture medium were removed and analyzed for differences in Bm-ipgm gene-specific transcript levels by qRT-PCR. The relative amounts of iPGM amplicon were determined by using the comparative ΔCT method and normalizing against the endogenous control gene (Bm-tub-1). The median value of the control group was set to 100% and the reduction in expression in the treated groups was calculated as a percentage of the control. The criterion for statistical significance between the results was carried out by ANNOVA and was expressed in the form of p value that was considered significant and marked as p <0.05 *, p <0.01 as highly significant **, and p <0.001 *** as very highly significant. Data represent mean ± SE of percent reduction in mf counts from two independent experiments.