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Table 2 Nucleic acid detection of schistosomes and their corresponding applications

From: Nucleic acid detection in the diagnosis and prevention of schistosomiasis

Biomarkers

Method

Sample type

Sensitivity %

Specificity %

References

S. japonicum

 SjR2 (AF412221)

LAMP

human and rabbit serum

96.7

100

[7]

 SjR2 (AF412221)

PCR

rabbit serum

60

100

[24]

 SjR2 (AF412221)

Real-time PCR

rabbit serum

[38]

 SjR2 (AF412221)

Nested PCR

rabbit serum

[40]

 SjCHGCS19

Nested PCR

human and rabbit serum

97.67

96.07

[41]

 28 s rDNA(Z46504)

LAMP

snail tissue

[14]

 SjCHGC08270

Real-time PCR

Water

93

[16]

 5D gene

Nested PCR

adult worm and egg

[58]

 NADH I

Real-time PCR

human, pig, buffalo feces

[28, 51, 52]

 Mitochondria gene

PCR

adult worm

[60]

 ITS2

Real-time PCR

mouse feces, snail tissue

[61]

S. mansoni

 SM1-7 (M61098)

PCR

human feces, urine, serum and mice serum

96–100

88–91.2

[17, 18, 22, 35, 67, 70, 71]

 SM1-7 (M61098)

PCR

water and plankton

[15, 65]

 SM1-7 (M61098)

LAMP

snail tissue

[10]

 SM1-7 (M61098)

PCR-ELISA

human feces

97.4

85.1

[68, 69]

 SM1-7 (M61098)

Real-time PCR

human feces and serum

80

92.4

[8]

 Genomic DNA

Hybridization

snail tissue

[63]

 18 s rDNA

Nested PCR

snail tissue

[46]

 mitochondria gene

Multiplex PCR

snail tissue

[64, 66]

 Satellite DNA

LAMP

mice feces and serum

[72]

S. haematobium

 Dra I

PCR

adult worm, snail tissue, human brain and urine

98

82

[12, 13, 20, 44, 73]

 Dra I

Real-time PCR

human serum, urine, feces

96.6–100

93.6–94.4

[23]

 Dra I

LAMP

snail tissue

[10]

 Sh110

PCR

adult worm, snail tissue

[74]

 Sh73/Dra I

Multiplex PCR

adult worm, snail tissue

[45]

 NADH3

PCR

snail tissue

[55]

 ITS2 (DQ677661)

PCR

human urine

[33, 34]

Differential detection methods

 ITS2 (AF146037/8)

PCR-RFLP

adult worm and cercaria

[75]

 COX I

Multiplex PCR

adult worm

[76]

 005AAT/Dra I

PCR

adult worm

[77]

 Dra I/SM1-7

PCR

human urine, feces

99–100

71–100

[17]

 COX I

PCR

adult worm, human blood

[56, 78]

 Mitochondria gene

Copro-PCR

human feces

87.7

100

[79]

  1. “—”: no mention or no accurate data in paper