Nested-PCR assay for detection of Schistosoma japonicum infection in domestic animals

Table 1 Detection of S. japonicum DNA in DBFPs and sera from two artificially infected goats and 6 artificially infected buffaloes

Animal	Days post-infection	DBFP		Serum
Animal	Days post-infection	No. of samples^a	No. of positive samples	No. of samples^a	No. of positive samples
Goat	3–4	4	4	4	0
	7–22	8	8	8	8
	60–158^b	20	20	20	20
	163–170^c	4	4	4	4
	172-175^c	4	4	4	0
	Total	40	40	40	32
Buffalo	3	6	6	6	0
	7-28	18	18	18	18
	Total	24	24	24	18

^a multiple samples collected from two goats and 6 buffaloes at different time points are collectively presented here ;^bThe samples were positive by using MHT; ^cThe samples were collected on days 25 to 37 post-treatment when the MHT results were negative but male worm infection examined by perfusion on final day

ISSN: 2049-9957