Utilizing the ultrasensitive Schistosoma up-converting phosphor lateral flow circulating anodic antigen (UCP-LF CAA) assay for sample pooling-strategies

Table 1 UCP-LF CAA assay formats for urine analysis

Assay format	Analyzed volume (μl)	TCA extraction solution volume (μl) / % (w/v)	Amicon (centrifugal) concentration device	QC threshold ¹ singlet test (pg/ml)
UCAA10	10 μl	10 μl / 4%	none	10
UCAA250	250 μl	250 μl / 4%	0.5 ml Filter Tube	1
UCAA2000	2 000 μl	2 000 μl / 4%	4 ml Filter Tube	0.1
UCAA14000	14 000 μl	1 000 μl / 30%²	15 ml Filter Tube³	0.03
UCAA40000	40 000 μl	10 000 μl / 10%²	50 ml Cell³	0.01

¹Quality control threshold (CAA concentration) for single test with wet reagents [20]
²Utilizing relevantly higher TCA concentration than the 4% (w/v) applied in the standard formats
³The 15 ml filter tube and 50 ml cell concentration is a two-step procedure requiring an additional 0.5 ml filter tube to concentrate down to 20 μl

ISSN: 2049-9957