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Table 3 Pooling strategy maintaining ultimate (UCAA2000) sensitivity

From: Utilizing the ultrasensitive Schistosoma up-converting phosphor lateral flow circulating anodic antigen (UCP-LF CAA) assay for sample pooling-strategies

 

Number of pools

Number of positive individuals

Individuals

pool size = 35

Prevalence 5%

Prevalence 1%

Prevalence 0.2%

35

1

2

1

1

70

2

4

1

1

140

4

7

2

1

280

8

14

3

1

560

16

28

6

2

1 120

32

56

12

3

 

Number of pools

Number of positive pools

Individuals

pool size = 35

Prevalence 5%

Prevalence 1%

Prevalence 0.2%

35

1

1 (100%)

1 (100%)

1 (100%)

70

2

2 (100%)

1 (50%)

1 (50%)

140

4

4 (100%)

2 (50%)

1 (25%)

280

8

8 (100%)

3 (37.5%)

1 (12.5%)

560

16

16 (100%)

6 (37.5%)

2 (12.5%)

1 120

32

32 (100%)

12 (37.5%)

3 (9.4%)

 

Number of pools

Number of individuals not requiring an individual test

Individuals

pool size = 35

Prevalence 5%

Prevalence 1%

Prevalence 0.2%

35

1

0

0

0

70

2

0

35

35

140

4

0

70

105

280

8

0

175

245

560

16

0

350

490

1 120

32

0

700

1 015

  1. Relation between prevalence and number of negative pools and samples not requiring an individual test results. At low prevalence the number of negative pools increases, hence less individuals require an individual result implying relevant reduction of the number of tests. Calculations are based on equal distribution of the ‘CAA-positive’ individuals, the least favourable condition leading to the highest number of individual tests. In real pooling applications, the number of negative pools expectedly will be higher than shown the above example due to non-random distribution of the ‘CAA-positive’ individuals