Skip to main content

Table 4 Pooling experiment with urine samples from River Basin children

From: Utilizing the ultrasensitive Schistosoma up-converting phosphor lateral flow circulating anodic antigen (UCP-LF CAA) assay for sample pooling-strategies

Study site

River Basin, Senegal

Included

81 children (7–12 years of age) with high susceptibility of being infected

Prevalence before PZQ

89% (72 children) based on individual UCAA10 diagnosis

 

100% (81 children) based on individual UCAA250 diagnosis

CAA level 1

519 pg/ml

Prevalence after PZQ

43.2% (35 children) based on individual UCAA10 diagnosis

 

86.4% (65 children) based on individual UCAA250 diagnosis

CAA level 1

33 pg/ml

POOLING

Pooling of samples collected after PZQ treatment

Pool composition

Nine pools, each comprising an equal volume of 9 children –‘9 sample pool’

One pool comprising an equal volume of all 81 samples – ‘81 sample pool’

Applied test

Pools were analysed with the UCAA250 format

CAA level 9-sample pool 1

47, 84, 29, 57, 60, 36, 22, 40, 33 pg/ml – average of nine ‘9 sample pools’ 45 pg/ml

CAA level 81-sample pool 1

40 pg/ml

  1. 1All concentrations indicate the average amount of CAA per individual, either of the whole selection (n = 81) or for the number of individuals in a specific pool