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Table 2 Case report forms (CRFs) for the entomologic studies

From: Development of a data collection and management system in West Africa: challenges and sustainability

Entomology CRF # and Title

Procedures Performed

Information Obtained

CRF 1: CSP Testing and blood Meal ELISA testing

ELISA testing for CSP antigen and human red cell antigens

Mosquitoes positive for malaria parasites or human red cells

CRF 2: Human landing catches, US CDC light traps

Estimate nightly and monthly anopheline biting rates

Number of infectious bites per person per month (EIR)

CRF 3: Ovarian dissections of captured Anopheline mosquitoes

Dissections performed using stereoscopic microscopes

Distinguish fed, unfed, half-gravid and gravid mosquito vectors

CRF 4: Mosquito species, Molecular forms and resistances

Use PCR to identify Anopheles species and kdr resistances

Separate gambiae from funestus and distinguish kdr genotypes

CRF 5: Pyrethrum spray catches (PSCs) and analyses

Pyrethroid spraying of houses slept in the night before

PSC estimate of nightly biting rate from # of fed mosquitoes/house

  1. CSP Circumsporozoite protein, ELISA Enzyme linked immunosorbent assay, PCR Polymerase chain reaction