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Table 1 Details of the multiplex qPCR primers and the probes used in the study

From: Co-parasitism of intestinal protozoa and Schistosoma japonicum in a rural community in the Philippines

Parasite

Gene target

GenBank Accession #

Reference

Primer / probe

Sequence (5′ → 3′)

Product size (bp)

Final concentration (nmol/L)

Blastocystis spp.

SSU rRNA

AY244621

[41]

Forward primer

GGTCCGGTGAACACTTTGGATTT

119

350

Reverse primer

CCTACGGAAACCTTGTTACGACTTCA

 

350

Probe

FAM-TCGTGTAAATCTTACCATTTAGAGGA-MGBNFQ

 

120

Entamoeba histolytica

SSU rRNA

X75434.1

[30, 71]

Forward primer

AACAGTAATAGTTTCTTTGGTTAGTAAAA

135

200

Reverse primer

CTTAGAATGTCATTTCTCAATTCAT

 

200

Probe

ROX-ATTAGTACAAAATGGCCAATTCATTCA-IBRQ

 

80

Giardia duodenalis

SSU rRNA

M54878.1

[30]

Forward primer

GACGGCTCAGGACAACGGTT

63

200

Reverse primer

TTGCCAGCGGTGTCCG

 

200

Probe

CY5-CCCGCGGCGGTCCCTGCTAG-IBRQ

 

100

Cryptosporidium spp.

COWP

AF248743.1

[30]

Forward primer

CAAATTGATACCGTTTGTCCTTCTG

150

300

Reverse primer

GGCATGTCGATTCTAATTCAGCT

 

300

Probe

HEX-TGCCATACATTGTTGTCCTGACAAATTGAAT-IBFQ

 

75

  1. SSU rRNA Small subunit ribosomal RNA, COWP Cryptosporidium oocyst wall protein