Fig. 2
From: Armigeres subalbatus is a potential vector for Zika virus but not dengue virus
The artificial urine infection of ZIKV or DENV-2 in the larvae of Ar. subalbatus. A Schematic diagram of the artificial virus urine infection experiment. B Susceptibility of different instar larvae reared in artificial urine contained ZIKV, and virus was detected in the fourth instar larvae. C RNA copies of ZIKV in infected midguts, ovaries, and salivary glands of adults. The results are expressed as the means ± standard errors (SEs). D The third instar larvae were reared in artificial urine with ZIKV, and the infection of larvae was detected at 1, 2, 3, 4 days post inoculation (dpi) with RT-PCR and RT-qPCR. E ZIKV was detected in midgut, anal papillae and carcass of fourth instar larvae at 4dpi with RT-PCR and RT-qPCR. F Virus particles were detected with ZIKV NS1 protein antibody (Thermo Fisher) by IHC and are displayed as obvious brownish-red marked by red arrow